A simple, precise, accurate and reliable HPTLC method has been developed and validated for analysis of Eperisone hydrochloride (EPE) and Diclofenac sodium (DIC) in their combined dosage form. Identification and analysis were performed on 100 mm x 100 mm layer thickness 0.2 mm, pre-coated silica gel G60-F254 aluminum sheet, prewashed with methanol and dried in an oven at 50°C for 5 min. Toluene : methanol : ethyl acetate : ammonia (6:2:2:0.05) (v/v/v/v) was used as mobile phase. Calibration plots were established showing the dependence of response (peak area) on the amount chromatographed. The validated calibration ranges were 450-1950 ng/spot and 300-1300 ng/spot for EPE and DIC with correlation coefficient (R2) 0.9958 and 0.9984 respectively. Average % recovery was between 99.55–100.11% and 99.09–102.41% for EPE and DIC respectively. The spots were scanned at 255 nm in a reflectance mode. The proposed method has been validated as per ICH guidelines and successfully applied to the estimation of EPE and DIC in their combined capsule dosage form.
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